Journal: bioRxiv

Article Title: Novel ACE2-Independent Carbohydrate-Binding of SARS-CoV-2 Spike Protein to Host Lectins and Lung Microbiota

doi: 10.1101/2020.05.13.092478

Figure Lengend Snippet: Binding of SARS-CoV-2 spike protein to human C-type lectins. Human C-type lectins ELISA results: wells were coated with the viral spike protein and the binding to different C-type lectins was evaluated in a calcium-containing buffer. The experiment has been performed three times in duplicate with similar results, and data were normalized over signal from BSA-coated wells. Error bars indicate standard deviations. OD: Optical density.

Article Snippet: a solution of 50 µL of SARS-CoV-2 spike protein (2019-nCoV Spike RBD-His Recombinant Protein, Cat: 40592-V08H, expressed in HEK293 cells, purchased from Sinobiological) at 5 µg/mL, in PBS (10mM, pH=7.4), were used to coat the Nunc MaxiSorp plate 2h at 37°C.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Novel ACE2-Independent Carbohydrate-Binding of SARS-CoV-2 Spike Protein to Host Lectins and Lung Microbiota

doi: 10.1101/2020.05.13.092478

Figure Lengend Snippet: Binding of SARS-CoV-2 spike protein to human Siglecs. Human Siglecs ELISA results: wells were coated with the viral spike protein and the binding to different Siglces was evaluated. The experiment has been performed three times in duplicate with similar results, and data were normalized over signal from blocking buffer-coated wells. Error bars indicate standard deviations. OD: Optical density.

Article Snippet: a solution of 50 µL of SARS-CoV-2 spike protein (2019-nCoV Spike RBD-His Recombinant Protein, Cat: 40592-V08H, expressed in HEK293 cells, purchased from Sinobiological) at 5 µg/mL, in PBS (10mM, pH=7.4), were used to coat the Nunc MaxiSorp plate 2h at 37°C.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Blocking Assay

Journal: bioRxiv

Article Title: Novel ACE2-Independent Carbohydrate-Binding of SARS-CoV-2 Spike Protein to Host Lectins and Lung Microbiota

doi: 10.1101/2020.05.13.092478

Figure Lengend Snippet: Binding of SARS-CoV-2 spike protein to different S. pneumoniae CPS. Wells were coated with different S. pneumoniae CPS and the binding to the viral spike protein was evaluated exploiting the mouse-Fc tag on the recombinant chimera spike protein. The experiment has been performed three times in duplicate with similar results, and data were normalized over signal from blocking buffer-coated wells. Error bars indicate standard deviations. OD: Optical density.

Article Snippet: a solution of 50 µL of SARS-CoV-2 spike protein (2019-nCoV Spike RBD-His Recombinant Protein, Cat: 40592-V08H, expressed in HEK293 cells, purchased from Sinobiological) at 5 µg/mL, in PBS (10mM, pH=7.4), were used to coat the Nunc MaxiSorp plate 2h at 37°C.

Techniques: Binding Assay, Recombinant, Blocking Assay

Journal: bioRxiv

Article Title: Novel ACE2-Independent Carbohydrate-Binding of SARS-CoV-2 Spike Protein to Host Lectins and Lung Microbiota

doi: 10.1101/2020.05.13.092478

Figure Lengend Snippet: Binding of SARS-CoV-2 spike protein to different LPS. Wells were coated with different LPS and the binding to the viral spike protein was evaluated exploiting the mouse-Fc tag on the recombinant chimera spike protein. The experiment has been performed three times in duplicate with similar results, and data were normalized over signal from blocking buffer-coated wells. Error bars indicate standard deviations. OD: Optical density.

Article Snippet: a solution of 50 µL of SARS-CoV-2 spike protein (2019-nCoV Spike RBD-His Recombinant Protein, Cat: 40592-V08H, expressed in HEK293 cells, purchased from Sinobiological) at 5 µg/mL, in PBS (10mM, pH=7.4), were used to coat the Nunc MaxiSorp plate 2h at 37°C.

Techniques: Binding Assay, Recombinant, Blocking Assay